Abstract
A simple and sensitive assay for polyamino acids (e.g., poly-L-glutamic and poly-L-aspartic acids) is described. The assay is performed by titrating anionic polyamino acids with a poly-L-arginine titrant. Earlier reported lower molecular weight titrants, such as protamine, did not interact with the highly negatively charged polyamino acids, and thus were not suitable for this analysis. The increase of poly-L-arginine concentration at the end point is monitored by utilizing a polycation-sensitive membrane sensor as an indicator electrode in a potentiometric set-up. Polyamino acids such as poly-L-glutamic and poly-L-aspartic acids are titrated down to 5 µg/mL with large potential changes at the end point (ca. 160 mV). The effect of the polyamino acid molecular weight and pH of the sample solution are also described. The method is applied for the evaluation of poly-L-glutamic acid functionalized cellulosic membranes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.