Abstract
Abstract In this paper, a novel and sensitive electrochemical aptasensor for detecting tetracycline (TET) with prussian blue (PB) as the label-free signal was fabricated. A PB-chitosan-glutaraldehyde (PB-CS-GA) system acting as the signal indicator was developed to improve the sensitivity of the electrochemical aptasensor. Firstly, the PB-CS-GA was fixed onto the glass carbon electrode surface. Then, colloidal gold nanoparticles (AuNPs) were droped onto the electrode to immobilize the anti-TET aptamer for preparation of the aptasensor. The stepwise assembly process of the aptasensor was characterized by cyclic voltammetry (C-V) and scanning electron microscope (SEM). The target TET captured onto the electrode induced the current response of the electrode due to the non-conducting biomoleculars. Under the optimum operating conditions, the response of differential pulse voltammetry (DPV) was used for detecting the concentration of TET. The proposed aptasensor showed a high sensitivity and a wide linear range of 10−9 ∼ 10−5 M and 10−5 ∼ 10−2 M with the correlation coefficients of 0.994 and 0.992, respectively. The detection limit was 3.2×10−10 M (RSD 4.12%). Due to its rapidity, sensitivity and low cost, the proposed aptasensor could be used as a pre-scanning method in TET determination for the analysis of livestock products.
Highlights
Tetracyclines (TCs) have been extensively used as a bacteriostatic and antibiotic drug in livestock production to control bacterial infections and to increase the growth rate of animals owing to its broad-spectrum antibacterial activity [1]
We proposed a novel label-free electrochemical aptasensor based on prussian blue (PB) as the signal for the determination of TET
The aptasensor was constructed by covalently attaching an amino-modified anti-TET aptamer on the AuNPs/PB-CS-GA coated glass carbon electrode (GCE) surface
Summary
Tetracyclines (TCs) have been extensively used as a bacteriostatic and antibiotic drug in livestock production to control bacterial infections and to increase the growth rate of animals owing to its broad-spectrum antibacterial activity [1]. AuNPs serving as the second layer modificatory material were used for two purposes: (a) to further accelerate the electron transfer (b) to bond and immobilize the anti-TET aptamer onto the electrode via Au-N bond. TET was captured by the anti-TET aptamer immobilized AuNPs/PB-CSGA modified electrode, which was folded into specific three-dimensional structures.
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