Electrochemical and Spectral studies on the Interaction of the Antiparasitic Drug Nitazoxanide with DNA

Abstract

The interaction of the antiparasitic drug nitazoxanide (NTZ) with salmon sperm double strand deoxyribonucleic acid (ss-ds-DNA) has been studied using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) at screen printed carbon electrode (SPCE). NTZ shows two irreversible oxidation peaks measured in 0.02M phosphate buffer (pH 4.0 and 7.4). UV/Vis spectroscopy was also employed to probe the interaction between NTZ and ss-ds-DNA. From the CV data, the diffusion coefficients were found as 5.1×10−7 and 4.9×10−9 cm2s−1 for NTZ and NTZ-DNA, respectively. Based on the CV and spectroscopic results, the binding constant between NTZ and DNA was calculated to be 4.1×105M−1 and 5.0×105M−1, respectively. The interaction as a sum of intercalation and groove binding modes was concluded. A procedure for the differential pulse voltammetric determination of NTZ at a screen-printed electrode surface modified with the ss-ds-DNA layer was also described. The method was applied for the determination of NTZ in spiked serum.