Abstract
The variation with time of photocurrents generated by bacteriorhodopsin in the form of purple membrane fragments (PMF) incorporatated in a B.L.M. are recorded. These generally increase at first and then decrease down to positive or negative values. The shape of the curves depend on the light energy, the value of the transmembrane electrical field and the pH of the aqueous solutions. The results are interpreted both on the basis of an initial active transport of protons across the purple membrane fragments as a result of the illumination and passive fluxes of part of the protons which have been transported. A general treatment is proposed to account for the experimental results based on an application of electrochemical kinetics to the proton transfer.
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