Electrocatalytic quantification of thrombin inhibitor dabigatran etexilate in solubilized system

Abstract

A new graphene-cerium dioxide nanocomposite-modified glassy carbon electrode (GR/CeO2/GCE)-based sensor has been developed for sensitive voltammetric quantification of thrombin inhibitor anticoagulant drug dabigatran etexilate (DAB) in solubilized system of Triton X-100. The voltammetric studies of DAB at GR/CeO2/GCE sensor exhibits a well defined cathodic peak for its reduction in Britton Robinson (BR) buffer at pH 3.8 in 0.001 % Triton X-100. The morphological characteristics of the developed GR/CeO2/GCE sensor were studied by scanning electron microscopy (SEM). The influence of optimization parameters such as pH, effect of different solvents, critical micelle concentration, and effect of loading of composite mixture of GR and CeO2 at GCE on electrocatalytic performance of the developed sensor was evaluated. Under optimized experimental conditions, the squarewave reduction peak current was linear over the concentration range of 5 (ng mL−1) to 5 (μg mL−1) with a correlation coefficient (r 2) of 0.9968 with detection limit (LOD) and quantification limit (LOQ) of 12.480 ng mL−1 and 41.602 ng mL−1 respectively with good stability and an acceptable reproducibility of 3.62 % relative standard deviation. The electrochemical quantification of DAB at GR/CeO2/GCE sensor holds great promise for its highly selective, sensitive screening and use of less hazardous Triton X-100 as solubilizing media.