Abstract
The oxidation of NADH on electropolymerizing methylene green (MG)-modified glassy carbon electrode (GCE) is described. The modified electrode shows an excellent electrocatalytic activity toward NADH oxidation, reducing its overpotential by about 650 mV and exhibits a wide linear range of 5.6–420 μM NADH with the detection limit of 3.8 μM. The electrode displays a good reproducibility and stability and the coexisting species does not affect the determination of NADH. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this electrode. MG-modified GCE can not only be used to detect NADH in biochemical reaction, but also can be used as the potential matrix of the construction of dehydrogenases biosensor.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
