Electroblotting of proteins from polyacrylamide gels for chemical characterization.

Abstract

AbstractSince the first complete genome sequence, that of the bacterium Hemophilus influenzae, was published in 1995 (1), a flurry of activity has seen the completion of the genomic sequences for more than 80 prokaryotic and eukaryotic organisms. Early in 2001 a major milestone was reached with the completion of the human genome sequence (2,3). A major challenge in the post-genome era will be to elucidate the biological function of the large number of novel gene products that have been revealed by the genome-sequencing initiatives, to understand their role in health and disease, and to exploit this information to develop new therapeutic agents. The assignment of protein function will require detailed and direct analysis of the patterns of expression, interaction, localization, and structure of the proteins encoded by genomes; the area now known as & proteomics (4). KeywordsBlotting MembraneProteomics ProjectLaser Desorption Mass SpectrometryInternal Amino Acid SequenceSubsequent Chemical AnalysisThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.