Electroantennogram responses of Batocera horsfieldi (Hope) to the selected volatile components of host plants, Rosa cymosa Tratt. and Rosa multiflora Thunb.

Abstract

To screen for host volatile components for effectively monitoring and trapping Batocera horsfieldi Hope (Coleoptera: Cerambycidae), Headspace Solid Phase Microextraction (HS-SPME) and Gas Chromatograph-Mass Spectrometry (GC-MS) were combined to analyze volatiles emitted from Rosa cymosa Tratt. and Rosa multiflora Thunb. The results showed that alcohols (55.519%) and aldehydes (81.387%) were the primary chemicals emitted by R. cymosa and R. multiflora , respectively. An electroantennogram response (EAG) test was used to compare and analyze the responses of male and female beetles to each of the selected fifteen components, and the results showed that only nine of these components triggered significant EAG responses. Sex and volatile concentration-specific responses were observed in B. horsfieldi . Male adults showed the strongest EAG response to 1-octen-3-ol, while female adults expressed the most powerful EAG responses to salicylaldehyde and linalool. These results indicated that the sensitivity of B. horsfieldi to host volatile components was different, and the EAG response was positively correlated with the concentration of the volatile components in a certain range. Male and female adults showed different preferences for the volatile components. Furthermore, B. horsfieldi showed different preferences for volatile components and exhibited diverse recognition reactions to specific functional groups and homologous isomers. This work provided favorable data support for the development of insect attractant, and laid a theoretical foundation for the local forestry ecological protection. • R. cymosa and R. multiflora mainly contain aldehydes and alcohols. • EAG response had positive correlation with concentration. • B. horsfieldi could identify different functional groups and homologous isomers. • Male and female showed different preferences for volatile components.