Abstract
Problem statement: The performance characteristics of Sensitive Screen-Printed (SPE) and Carbon Paste (CPE) electrodes were investigated for the Determination of Doripenem (DP) in pure, pharmaceutical preparations and biological fluids. Approach: The proposed electrodes is characterized in terms of plasticizer type, response time, pH and temperature. Results: The two electrodes showed nearly Nernstian behaviours over the concentration range of 2×10−4-5×10−2 mol/l of the drug with slopes of 58 and 57 mV/decade for SPE and CPE electrodes, respectively. The electrodes exhibited good selectivity for DP with respect to a large number of inorganic cations and organic substances present in the biological fluids. The method was precise, as shown by the mean recoveries of 99.49-100 and 98.49-99.49% with mean relative standard deviations 0.38-0.78 and 0.60-0.90% for SPE and CPE electrodes, respectively. Conclusion: Doripenem was determined successfully in pure solutions, in vials or in biological fluids using the standard addition and potentiometric titration methods.
Highlights
Doripenem (DP) (S-4661) (Fig. 1) is a recently developed member of the carbapenem class of betalactam antibiotics
To meropenem and ertapenem, but unlike imipenem, doripenem has a 1-βmethyl side chain that provides resistance to the renal enzyme I-dehydropeptidase. It was approved by the US Food and Drug Administration (FDA) in 2007 for the management of patients with complicated intraabdominal infections and complicated Urinary Tract Infections, including pyelonephritis (Mori et al, 1996)
Doripenem has been determined in its pharmaceutical formulation and in plasma using different techniques, including spectrophotometry (Piontek and Ska, 2010) chromatography High Performance Liquid Chromatography (HPLC) with various detection methods (Sutherland and Nicolau, response, applicability to coloured and turbid solutions and possible interface with automated and computerised systems (Santini et al, 2008)
Summary
Because these sensors offer the advantage of simple design and operation, reasonable selectivity, fast. Doripenem has been determined in its pharmaceutical formulation and in plasma using different techniques, including spectrophotometry (Piontek and Ska, 2010) chromatography High Performance Liquid Chromatography (HPLC) with various detection methods (Sutherland and Nicolau, response, applicability to coloured and turbid solutions and possible interface with automated and computerised systems (Santini et al, 2008) For these advantages, ISEs have found various applications: in clinical chemistry, environmental protection, water, soil and analytical chemistry in general (Kormosh et al, 2008). The method described here is based on the ion-pair formation between doripenem and sodium tetraphenyl borate as electroactive materials and Tricresyl Phosphate (TCP) as a placticiser in the doripenem matrix These sensors exhibited analytical characteristics with near-Nernstian sensitivity and low detection limit and were useful as indicator electrodes in potentiometric titrations of doripenem in pure form, in vials, in urine and in serum samples.
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