Electroanalysis of lincomycin using boron-doped diamond thin film electrode applied to flow injection system

Abstract

The electroanalysis of lincomycin was investigated using boron-doped diamond thin film (BDD) electrodes. First, the electrochemistry of lincomycin was studied by cyclic voltammetry as a function of pH of the solution, scan rate and the concentration of lincomycin. Comparison experiments were carried out using glassy carbon electrode. Boron-doped diamond thin film electrode provided well-resolved oxidation irreversible cyclic voltammogram. It was found that the peak position was 1.2 V (versus Ag/AgCl reference electrode). Second, the amperometric detection with BDD electrode was coupled with the flow injection analysis for the determination of lincomycin. The linear range of 0.5–125 μM and the detection limit of 0.02 μM were obtained. The percent recovery was shown in a range of 96–103. This purposed method was also applied to a drug formulation sample. It was found that the results (293 mg mL −1) were comparable to those labeled (300 mg mL −1).