Abstract
Microbial fuel cell (MFC) is an alternative approach in generating renewable energy by utilising bacteria that will oxidize organic or inorganic substrates, producing electrons yielded as electrical energy. Different species of exoelectrogenic bacteria capable of generating significant amount of electricity in MFC has been identified, using various organic compounds for fuel. Soil sample taken from rice paddy field is proven to contain exoelectrogenic bacteria, thus electricity generation using mixed culture originally found in the soil, and pure culture isolated from the soil is studied. This research will isolate the exoelectrogenic bacterial species in the rice paddy field soil responsible for energy generation. Growth of bacteria isolated from the MFC is observed by measuring the optical density (OD), cell density weight (CDW) and viable cell count. Mixed bacterial species found in paddy field soil generates maximum power of 77.62 μW and 0.70 mA of current. In addition, the research also shows that the pure bacterium in rice paddy field soil can produce maximum power and current at 51.32 μW and 0.28 mA respectively.
Highlights
Microbial Fuel Cell (MFC) is a device, which enables generation of electricity by exploiting bacterial action in metabolizing organic or inorganic substrate under anaerobic condition
Soil sample taken from rice paddy field is proven to contain exoelectrogenic bacteria, electricity generation using mixed culture originally found in the soil, and pure culture isolated from the soil is studied
Growth of colony 2 on nutrient agar plate halted at 8th hour after streaking, colony 2 was not considered for inoculation in pure culture Microbial fuel cell (MFC)
Summary
Microbial Fuel Cell (MFC) is a device, which enables generation of electricity by exploiting bacterial action in metabolizing organic or inorganic substrate under anaerobic condition. The breakdown of the process occurring is that in the anodic chamber, substrate is oxidized by bacteria, producing electrons that are transferred to anode electrode. The electrons will be further transported to the cathode via resistor. Current is generated by the positive terminal and flows to the anode, in contrast to the direction of electron flow [1, 2]. There are several designs of MFC currently being used, the single chamber MFC, dual or two chambers MFC and triple chambers MFC [3]. Substrate is filled into the vessel, sandwiched between the two electrodes
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