Electrically stimulated hydroxyapatite–barium titanate composites demonstrate immunocompatibility in vitro

Abstract

For fabricating the filler materials for tissue re-growth, the primary concern should be focused on the immunocompatibility of the material. Lymphocyte T-cells and macrophages activate each other and induce immune suppressive effects. Polarized hydroxyapatite–barium titanate composite surfaces showed comparatively less macrophage activity than unpolarized surfaces. Lymphocytes isolated from human umbilical cord blood were cultured separately on both polarized and unpolarized samples. The macrophage with nitric oxide and myeloperoxidase showed less activity on polarized composite surfaces as compared to lipopolysaccharide of stimulated cells from which it can be inferred that the polarized composites showed immunocompatibility for which these materials can be used a preferred material in tissue engineering applications.