Abstract
In this paper, we described a miniaturized microfluidic device able to electro-loading small volumes (200nl) of biologic samples via ElectroWetting on Dielectric (EWoD) actuation and detect DNA via polymerase chain reaction (PCR). The miniaturized silicon-based chip contains 48 microchambers properly chemically treated for EWOD process. The device was designed to contain also integrated temperature sensors and heaters for the thermal cycling of PCR (Polymerase Chain Reaction) reaction. The system here proposed is a very appealing potential solution for genetic point-of-care (PoC) applications.
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