Electrical stimulation of cell growth on layers of composite material based on carbon nanotubes and polymers

Abstract

A method of electrical stimulation of the growth of fibroblast cells and mesenchymal stem cells cultured on layers of a composite material deposited from water dispersion of carbon nanotubes and polymers (serum albumin, chitosan) and cured by laser method is proposed. A system for electrical stimulation has been developed that allows electrical stimulating pulses to be transmitted to cells through electrically conductive layers of a composite material. Electrical stimulation was carried out for 48 h with pulsed electric signal (80 mV amplitude, 1 ms pulse duration, 0.5 s pause). As a result of electrical stimulation, an increase in the density of cell distribution over the surface of the layers of the composite material and formation of a cell monolayer were observed.