Abstract
A micropuncture technique is described for isolated surface epithelial cells and oxyntic cells of Necturus gastric mucosa, and for intact isolated tubules from the same tissue. The potential obtained is similar to that found for the cells in the intact tissue, whereas the measured membrane resistance is about 100 times higher. This is explained by cell-cell coupling in the intact mucosa, and is confirmed by direct two-electrode experiments in the intact tissue or isolated tubule. The calculated tissue resistance is about twice as high as the measured resistance after allowance is made for glandular infolding, hence the possibility remains that there is a low resistance shunt across the tissue.
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