Electrical impedance spectroscopy (EIS) in plant roots research: a review

Gang Zhang,Yang Liu,Bao Di,ZhenHui Ren,Ji Qian,DongMing Li

doi:10.1186/s13007-021-00817-3

Gang Zhang, Yang Liu

Open Access

https://doi.org/10.1186/s13007-021-00817-3

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Journal: Plant Methods	Publication Date: Nov 13, 2021
Citations: 20	License type: open-access

Affiliation: Hebei Agricultural University, Institute of Software

Abstract

Nondestructive testing of plant roots is a hot topic in recent years. The traditional measurement process is time-consuming and laborious, and it is impossible to analyze the state of plant roots without destroying the sample. Recent studies have shown that as an excellent nondestructive measurement method, although electrical impedance spectroscopy (EIS) has made great achievements in many botanical research fields such as plant morphology and stress resistance, there are still limitations. This review summarizes the application of EIS in plant root measurement. The experiment scheme, instrument and electrode, excitation frequency range, root electrical characteristics, equivalent circuit, and combination of EIS and artificial intelligence (AI) are discussed. Furthermore, the review suggests that future research should focus on miniaturization of measurement equipment, standardization of planting environment and intelligentization of root diagnosis, so as to better apply EIS technology to in situ root nondestructive measurement.

Highlights

The root is a vital organ of a plant
Electrical Impedance Spectroscopy (EIS) technology makes it possible to measure roots in situ, and the non-invasive nature of this technology applies to all types of plant tissues
Technical details of the measurement Through the analysis and research of literature, we found that the measurement technical details can determine the accuracy and rationality of the measurement data, which is mainly manifested in the following aspects:

Summary

Introduction

The root is a vital organ of a plant. It is responsible for anchorage, obtaining nutrients in the soil, and improving soil composition [1]. The electrical impedance method, a new technology developed in recent years, measures the root electrical characteristics (Capacitance, Resistance, and EIS) in the circuit under an external power supply of a certain frequency. In 2019, at a low frequency (1 kHz), I Cseresnyes applied the parameters of capacitance, impedance phase Angle and conductance together for the first time to in situ monitor the root condition of potted wheat [31].

Results

Conclusion