Abstract
In this contribution triggering of the acetylcholine receptor (AChR) in Neuro2a murine neuroblastoma (N2a) cells was studied by means of electrical impedance analysis. N2a cells were immobilized in a bactoagar gel matrix and stimulated with acetylcholine (ACh). Gel films were prepared in between the electrodes of a novel impedimetric sensor. Devices were integrated in a micro fluidic bioreactor for cell stimulation in flow-through regime. Shifts in the impedance spectra (10 to 110 MHz) of gels showed that AChR triggering is accompanied by an increase in the overall conductivity of the gel in a time scale of tens of minutes.
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