Abstract
The electrical conductivity test has been used to evaluate seed vigour in many species, especially legumes, such as peas, beans and soybeans. The objective of this research was to evaluate the effect of temperature and storage period on the results of the electrical conductivity test and the mineral composition of the imbibition solution of bean seeds (Phaseolus vulgaris L.). Three seed lots of the cultivar IAPAR - 81 and cultivar IPR - TIZIU were stored at 10, 25 and 25-10º C (six months at 10º C and six months at 25º C) The following evaluations were made every three months for a year: seed water content, germination and vigour (accelerated ageing and electrical conductivity) and the potassium, calcium and magnesium contents of the imbibition solution. The cultivars showed similar behavior in storage. The reduction in the vigour of bean seeds stored at 10º C was verified by accelerated ageing and electrical conductivity tests. The bean seed storage at low temperature (10º C) does not influence the results of the evaluation of seed vigour by electrical conductivity test and the quantification of calcium, magnesium and potassium ions. The evaluation of vigour by the electrical conductivity test is not recommended for bean seed stored by long periods (above 9 months).
Highlights
An evaluation of seed vigour is important for identifying possible differences in potential performance between similar germination lots (MARCOS-FILHO, 1999)
Included among the most well-known vigour tests, electrical conductivity is recommended as a procedure for evaluating pea seed vigour and is suggested for soybean seeds
It appears promising as a means of standardization (HAMPTON; TEKRONY, 1995; VIEIRA; KRYZANOWSKI, 1999; MARCOS-FILHO; VIEIRA, 2009)
Summary
An evaluation of seed vigour is important for identifying possible differences in potential performance between similar germination lots (MARCOS-FILHO, 1999). These vigour tests, using direct or indirect methods, estimate the probable behavior of the seed when deterioration occurs, either based on the actual metabolic activity or of constituent parts of the seed (BAALBASKI et al, 2009). Included among the most well-known vigour tests, electrical conductivity is recommended as a procedure for evaluating pea seed vigour and is suggested for soybean seeds It appears promising as a means of standardization (HAMPTON; TEKRONY, 1995; VIEIRA; KRYZANOWSKI, 1999; MARCOS-FILHO; VIEIRA, 2009). Among the solutes leached into the imbibition solution are carbohydrates, fatty acids, amino acids, organic acids, proteins, phenolic compounds and K+, Ca2+, Na+ and Mg2+ ions (CUSTÓDIO; MARCOSFILHO, 1997; FESSELet al., 2006; PANOBIANCO;VIEIRA, 2007; VIEIRA et al, 2008)
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