Electrical and mechanical properties of the crustacean stretch receptor during sinusoidal length changes.

Abstract

Isolated slowly adapting stretch receptors of the crayfish (Astacus fluviatilis) were exposed to sinusoidal length changes. The mechanical force, the receptor current and the receptor potential were analysed in terms of frequency response (Bode plots) and input/output functions. Within the frequency range investigated (0.3 to 80 Hz) the mechanical force was found to be only slightly dependent on the frequency of the sinusoidal stimuli, the slope of the gain function in the Bode plot being close to zero. Dynamic length-force curves exhibited a characteristic hysteresis. In potential clamp experiments, the receptor current induced by sinusoidal length changes was dependent on the clamp potential attaining maximal amplitude at a membrane potential of about -20 to -30 mV for 80 Hz mechanical stimulation. The membrane potential at which the receptor current changed sign (the reversal potential) increased with increasing frequency. In Bode plots of the receptor current the gain was represented by a straight line, having a slope of about 1.2 dB/octave. The phase shift was positive at low frequencies. Bode plots of the receptor potential gain exhibited a characteristic peak in the region of 15 Hz. Below this peak the gain increased with about 1.2 dB/oct, above the peak the gain decreased with about 4.8 dB/oct. There was a small positive phase shift at low frequencies; at high frequencies the phase shift became negative. The impedance of the cell, as calculated from the receptor potentials and the current responses at resting membrane potential, gave Bode plots comparable to a simple resistive-capacitive filter compatible with the electrical properties of the receptor cell membrane. The cut-off frequency of this low pass filter decreased with decreasing stretch amplitude. One physiological significance of this finding, is that the performance of the receptor is improved at low levels of stimulation by reducing the bandwidth of the system.