Abstract
Purpose: To observe the effects of electric fields (EFs) on the migration of human retinal pigment epithelial (RPE) cells and to explore possible related mechanisms. Methods: Cultured human RPE cells were exposed to EFs, and images of the cells were obtained at regular intervals to evaluate the cell migration and viability. Distribution of F-actin and β 1 integrin was measured by immunohistochemistry. Expression of β 1 integrin was determined by PCR and Western blotting. Results: Exposure to EFs resulted in a cathodal-directed migration of RPE cells and a cathodal accumulation of F-actin and β 1 integrin in the cells. EF stimulation increased expression of β 1 integrin both in mRNA and protein levels. These effects could be inhibited by cytochalasin B. Conclusion: EFs induce directed migration of RPE cells, and this effect is, to some extent, regulated by the interaction between cytoskeleton and integrins.
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