Abstract
Protoplast fusion of Lactobacillus plantarum АТСС10012 was examined with an electric field fusion method using its double mutants having drug resistance and auxotrophic marker genes. Under the conditions of pulse width of 150µs and an electric field intensity of 6kV/cm, the frequency of obtained strains carrying the phenotypes from both mutants was highest (6.2 x 10 −7). The frequency was at least one order higher than the reversion frequencies and that of conjugation. These results suggest the completion of protoplast fusion.
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