ELDOR Spectroscopy Reveals that Energy Landscapes in Human Methionine Synthase Reductase are Extensively Remodelled Following Ligand and Partner Protein Binding

Abstract

ELDOR roadmap: ELDOR spectroscopy gives access to the energy landscape of an electron transfer protein. In human methionine synthase reductase (MSR) this landscape is rugged and is remodelled through effector ligand and protein binding. The mobility of flavin-binding domains suggests that large-scale motion underpins electron transfer across multiple redox cofactors in the MSR–methionine synthase protein complex (see figure).