Abstract
Assessment of the ex-vivo myocardial tissue and cellular stiffness may be directly applicable to early-diagnosis and treatment of the progression of myocardial infarction pre- and post- stem-cell therapy. This study quantifies the cellular elasticity of HL-1 (Claycomb) cardiomyocytes. HL-1 cells were thawed from a cryo-preserved state, cultured and reproduced until a stable line was maintained. Two separate batches were created and were maintained in the Claycomb medium, enriched with Fetal Bovine Serum, penicillin/streptomycin, norepinephrine and L-glutamine. They were subsequently studied under a QscopeTM 350 (Quesant Instrument Corporation, California, USA) AFM device, with sphere shaped cantilever tips (NanoAndMore GmbH, Germany) having a radius R=0.8 μm and a spring constant of k=0.05 N/m. The tip approaching speed ranged between 0.3-0.6 μm/s and the indentation depth was approximately 1.4 μm. The Young's modulus (E) for the elasticity was extracted after appropriate fitting of the AFM nanoidentation curves according to the Hertzian model. A mean cellular stiffness of E=1.55±0.15 kPa (n=50, mean±SE) was elicited compared to an epicardial value of ex-vivo sectioned LV tissue Eepi=14.7±0.8 kPa (from male C57BL/6 mice in Kreb's), published previously. Cellular elasticity measurements are demonstrated with a potential usefulness to myocardial stem-cell therapy post-myocardial infarction.
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