Elastic Image Registration to Quantify 3-D Regional Myocardial Deformation from Volumetric Ultrasound: Experimental Validation in an Animal Model

Abstract

Although real-time 3-D echocardiography has the potential to allow more accurate assessment of global and regional ventricular dynamics compared with more traditional 2-D ultrasound examinations, it still requires rigorous testing and validation should it break through as a standard examination in routine clinical practice. However, only a limited number of studies have validated 3-D strain algorithms in an in vivo experimental setting. The aim of the present study, therefore, was to validate a registration-based strain estimation methodology in an animal model. Volumetric images were acquired in 14 open-chest sheep instrumented with ultrasonic microcrystals. Radial strain (ɛRR), longitudinal strain (ɛLL) and circumferential strain (ɛCC) were estimated during different stages: at rest, during reduced and increased cardiac inotropy induced by esmolol and dobutamine infusion, respectively, and during acute ischemia. Agreement between image-based and microcrystal-based strain estimates was evaluated by their linear correlation, indicating that all strain components could be estimated with acceptable accuracy (r = 0.69 for ɛRR, r = 0.64 for ɛLL and r = 0.62 for ɛCC). These findings are comparable to the performance of the current state-of-the-art commercial 3-D speckle tracking methods. Furthermore, shape of the strain curves, timing of peak values and location of dysfunctional regions were identified well. Whether 3-D elastic registration performs better than 3-D block matching-based methodologies still remains to be proven.