Abstract
Brain explants from a Caribbean sharpnose shark ( Rhizoprionodon porosus) and six spiny dogfish ( Squalus acanthias) were cultured at 22 °C in Leibovitz L-15 medium, 1000 mOsmol, with 350 mM urea, 20 mM added NaCl, and either 2% fetal bovine serum, 2% bull shark ( Carcharhinus leucas) serum, or 2% spiny dogfish serum. Cell outgrowth from explants appeared after 1 month. Cells were subcultured once with 20% survival, and were viable at termination of the trials 6 months later. Cell morphology appeared normal throughout the experiment.
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