El daño peroxidativo en leucocitos peritoneales de ratones viejos disminuye si se suplementa la dieta con galletas enriquecidas con antioxidantes. Relación con la supervivencia

Abstract

Introduction ageing is associated with chronic oxidative stress, which leads to increased damage to macromolecules, such as lipids. One of the most important end products of lipid peroxidation is the malondialdehyde molecule (MDA). Leukocytes produce reactive oxygen species to support their functions and are highly sensitive to oxidative damage. The aim of this study was to ascertain MDA levels in peritoneal leucocytes from mice during ageing, as well as the effect of antioxidant dietary supplementation in aged mice on the levels of this molecule and its possible incidence on longevity. Material and methods MDA levels were assessed by highperformance liquid chromatography in peritoneal leukocytes from ICR (CD 1) mice at young (16 ± 2 weeks), adult (36 ± 2 weeks), mature (52 ± 2 weeks) and old (71 ± 2 and 81 ± 2 weeks) ages. At the latter age, MDA content was also measured after 5 and 15 weeks of dietary supplementation (20% w/w) with biscuits enriched with nutritional doses of antioxidants. The mice received the supplementation until death to ascertain the possible relationship between ingestion of an antioxidant-enriched diet and longevity. Results MDA levels increased during ageing, whereas the levels of lipid peroxidation decreased after 5 and 15 weeks of antioxidant dietary supplementation in aged mice. Moreover, an inverse relationship between MDA content and longevity was observed. Conclusions our results demonstrate that in aged mice ingestion of an antioxidant-enriched diet decreases lipid peroxidation in peritoneal leukocytes, leading to increased longevity.