Abstract
This study aims to determine the effect of citric acid-glucose based microwave assited extraction (MAE) method on total polyphenols content from (Peperomia pellucida [L.] Kunth) herbs. Dried samples were extracted using green solvent glucose citric acid with a ratio of 1: 1 g/g, 3: 1 g/g, and 5: 1 g/g, respectively, 30% microwave power for 5 minutes, and 1:5 g /mL solid-liquid ratio. Then the obtained extract solution was performed determination total polyphenols content using Folin-Ciocalteu reagent, and the absorbance was measured using a UV-Vis spectrophotometer at 791 nm. Based on the results, obtained total polyphenols content according to the extraction condition namely 214.405 mg GAE/g sample (3: 1 g /g solvent), 84.833 mg GAE /g sample (1: 1 g /g solvent) and 53,9451 mg GAE/g sample (5: 1 g /g solvent), respectively. This study is a preliminary data about the use of citric acid-glucose based microwave assisted extraction method on total polyphenols content from this plant quickly, easily and efficiently.
Highlights
Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan metode citric acid-glucose based microwave assited extraction (MAE ) terhadap ekstraksi polifenol total dari herba suruhan (Peperomia pellucida [L.] Kunth)
This study aims to determine the effect of citric acid-glucose based microwave assited extraction (MAE) method on total polyphenols content from (Peperomia pellucida [L.] Kunth) herbs
Application of natural deep eutectic solvents for extraction and determination of phenolics in Cajanus cajan leaves by ultra performance liquid chromatography
Summary
Aquades, asam galat, asam sitrat, folin-ciocalteu, glukosa, natrium karbonat. Alat yang digunakan adalah Mikrowave, batang pengaduk, corong kaca, kondensor, timbangan analitik, corong Buchner, labu ukur, gelas kimia, pipet tetes, spektrofotometer, cawan porselin, pipet ukur, propipet, software Design Expert Versi 10.0, Hot plate, Magnetik stirrer, Vortex mixer, kain saring, dan spatel
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