Abstract
Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-8 to enhance the inflamation reaction and MMP-8 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of MMP-8 and IL-8 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of MMP-8 and IL-8 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of MMP-8 and IL-8 are increasing during the periapical tissue damage that induced by E.faecalis.
Highlights
The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal
The analysis revealed that the expression of Matriks metalloproteinase (MMPs)-8 and IL-8 increased significantly in group B when E.faecalis was induced
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Summary
Jenis penelitian yang digunakan adalah eksperimental laboratoris. Sampel penelitian menggunakan tikus jantan yang diperoleh dari Fakultas Kedokteran Hewan Universitas Airlangga, dewasa, jantan, umur 8 - 12 minggu, berat badan antara 120 - 150 gram, dengan alasan perubahan berat badan selama penelitian relatif kecil, gigi molar sudah tumbuh sempurna, kondisi fisik sehat. Kelompok Perlakuan B dilakukan preparasi gigi hingga terjadi perforasi atap pulpa, pada hewan coba kelompok ini dilakukan injeksi 10 μl BHI-b berisi bakteri E.faecalis ATCC212 sebanyak 106 CFU dengan mikropipet. Setelah dipastikan telah terjadi kerusakan maka selanjutnya dilakukan pengecatan imunohistokimia menggunakan antibodi anti MMP-8 dan IL-8 agar dapat dilihat dengan perbesaran 1000x pada 20 lapang pandang dalam mikroskop cahaya untuk selanjutnya dilakukan perhitungan jumlah sel limfosit yang mengekpresikan MMP-8 dan IL-8 di jaringan periapikal.Untuk kebutuhan inferensial, maka data yang diperoleh dari hasil penelitian ini dianalisa dengan uji statistik, yaitu: Uji normalitas untuk melihat apakah mempunyai distribusi yang normal dilakukan dengan uji Kolmogorov-Smirnov Test, Uji homogenitas untuk melihat homogenitas variant berdasarkan populasi berat badan umurnya dengan uji Levene Test, dan Uji t-test satu arah untuk melihat adanya pengaruh induksi E.faecalis terhadap perbedaan jumlah sel MMP-8 dan IL-8 pada setiap kelompok kontrol (A) dan kelompok perlakuan (B). Data dikatakan tidak ada beda bila p > 0,05 dan ada beda bila p < 0,05
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