Abstract
Blood groupH substance prepared from pig gastric mucosa is a potent inhibitor of the haemagglutinating action ofWGA (wheat germ agglutinin). From the blood group substance, upon treatment with NaOH−NaBH4, aWGA-inhibiting oligosaccharide was detached, which was purified by gel filtration and paper chromatography and shown by gas chromatography to contain galactose, N-acetylglucosamine and N-acetylgalactosaminitol in a molar ratio of 2∶3∶1. Enzymatic release of a N-acetylglucosamine residue destroyed theWGA-inhibitory activity of the oligosaccharide. The importance of terminal N-acetylglucosamine residues for the interaction withWGA is discussed.
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Schedule a callMore From: Monatshefte für Chemie und verwandte Teile anderer Wissenschaften
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