Abstract

Oat seeds were germinated 72 h in the dark and thereafter cultivated in solutions with or without 10 − 3 M amitrole 72 h in the dark and 36 h in the light (3000 lx). Plants treated in this way become chlorotic (content of chlorophyll: 25% of the control) without reduction of weight. The chlorophyll suppression is connected with increased concentrations of amino acids and free ammonia. Slight differences in the degree of enhancement between the various amino acids are not specific for definite metabolic groups. In contrast to results with microorganisms, the biosynthesis of histidine is not inhibited by amitrole; an alternative pathway of histidine biosynthesis may exist in higher plants. The serine-glycine-pool is not depleted, that means that the reaction of amitrole with serine is not responsible for the amitrole chlorosis in oat plants. Some of the visible symptoms of amitrole toxicity may be due to the action of accumulated ammonia.

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