Abstract
The mechanism of potential tumor cell spread during laparoscopy is poorly understood. It is speculated that the increased intraabdominal pressure and the use of carbon dioxide during laparoscopy change the metastatic potential of carcinoma cells. The study investigated the influence of CO2 and helium insufflation and different pressure (10 mm Hg / 20 mm Hg) on the in vitro expression of E-Cadherin, CD44v6, ICAM-1 andβl-Integrin on HT-29 colon carcinoma cells. The adhesion molecule expression was measured 1, 12, 24, 48 and 96 h after CO2 and helium insufflation using flowcytometry (FACScan). HT-29 cell line showed a short significant decrease in E-Cadherin, CD44v6, ICAM-1 and βl-integrin expression after CO2 exposure. Helium insufflation had only influence on the expression of β1 -integrin. Whether these changes increase the metastatic potential of tumor cells in vivo needs further investigation.
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