Abstract
The mechanism of potential tumor cell spread during laparoscopy is poorly understood. It is speculated that the increased intraabdominal pressure and the use of carbon dioxide during laparoscopy change the metastatic potential of carcinoma cells. The study investigated the influence of CO2 and helium insufflation and different pressure (10 mm Hg / 20 mm Hg) on the in vitro expression of E-Cadherin, CD44v6, ICAM-1 andβl-Integrin on HT-29 colon carcinoma cells. The adhesion molecule expression was measured 1, 12, 24, 48 and 96 h after CO2 and helium insufflation using flowcytometry (FACScan). HT-29 cell line showed a short significant decrease in E-Cadherin, CD44v6, ICAM-1 and βl-integrin expression after CO2 exposure. Helium insufflation had only influence on the expression of β1 -integrin. Whether these changes increase the metastatic potential of tumor cells in vivo needs further investigation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
