Abstract
The incorporation of [ 3H]leucine in a great number of brain regions has been investigated quantitatively. An apparatus for the semiautomatic counting of silver grains is described, with surface illumination and minimal background remission. The values of activity in various brain areas were scanned on a grid with measuring diaphragm. The method is especially suitable for large scale routine scanning of great numbers of different brain areas. The rates of [ 3H]leucine incorporation are different in various brain areas. Lowest and highest levels of activity differ by a factor of 15. Within one area, the values are exactly reproducible. Good correlations could be found between rates of [ 3H]leucine incorporation and glia- and nerve cell volume density, succinate dehydrogenase, copper content, acid phosphatase and “supply of tissue” (product of capillary surface and blood quantity, ml × mm 2/min/mm 3 of fresh tissue). A hypothesis linking [ 3H]leucine incorporation to elementary biochemical processes on the cellular level is advanced.
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