Abstract
ABSTRACT The capacity of the corticosteroid-binding globulin (CBG) in serum was determined under equilibrium conditions based on the partition of cortisol between CBG, albumin and an adsorbent. A simplified mathematical model was used to calculate the optimal range of serum dilution, cortisol concentration and Florisil. To one ml of a 1:10 diluted serum 1 μg cortisol, a trace amount of 3H-cortisol and 40 mg Florisil are added. After reaching the equilibrium at 4°C the radioactivity of the supernatant is measured and the cortisol concentration is calculated. This procedure is repeated, but instead of the whole serum an albumin solution correspondent to the concentration in the individual sample is used. The CBG capacity is calculated by substracting the albumin bound from the whole serum bound cortisol fraction. The reliability of the proposed method was established by analysing the samples also by equilibrium dialyses. The correlation coefficient was 0.98, the regression line y = 0.97x. The average CBG capacity of normal males and females was 22.1 μg cortisol/100 ml (n = 13), of oestrogen treated females 49.6 (n = 7).
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