Ein verbessertes verfahren zur isolierung und quantitativen bestimmung von vincaleukoblastin aus intakten pflanzen und gewebekulturen von catharanthus roseus g. don. / An im proved method of isolation and titative determination of vincaleucoblastine from intact plants and tissue cultures of catharanthus roseus g. don.

Abstract

Improved methods of extracting vincaleucoblastin from leaves and tissue cultures of Catharanthus roseus and of the quantitative determination of this dimeric indole-alkaloid by reflectance densitometry of TLC-plates have been established. Approximately 0.05% VLB were found in the leaves and, using high-performance-thin-layer-chromatography, 0.00013% VLB in callus cultures (not in suspension cultures!) derived from this plant