Ein Beitrag zur Analyse der Gibberelline in Samen von Pisum sativum L.

Abstract

Unripe seeds of Pisum sativum were analyses for gibberellins and gibberellin-like substances. Forty kg of seeds were extracted with methanol, the methanol was evaporated and the residue distributed between water and ethylacetate, and between water and butanol at different pH-values. The acidic ethylacetate and the butanol fractions were separated by thin layer chromatography. In the acidic ethylacetate fraction a new substance tentatively named gibberellin Ax was isolated by preparative thin layer chromatography. In different solvent mixtures this substance migrates closely behind gibberellin A3. It is biological active in a dwarf pea and in dwarf maize mutant bioassays. IR and fluorescense spectra point to a gibban structure. Furthermore gibberellin A5 was found to be the main gibberellin in pea seeds. It was identified by its Rf-values and the Rf-values of its methylester in different solvent systems as well as by its characteristic relative biological activity in three biological test systems. Most probably gibberellin A6 is also present in the pea seeds, but because the amounts detected were small, no clear evidence can be presented. In the butanol phase two gibberellin-like substances could be separated by thin layer chromatography.