Abstract
Rat and mouse have become important animal models to study various human diseases such as cancer. Here, we use eggplant peel ethanolic extract to visualise chromosome banding. This extract is easy to prepare, a novel, available alternative. The colour of this extract is bright dark green colour. This stain gave us good result for show the chromosomes banding, we used Thin Layer Chromatography (TLC) examination, Infrared (IR) spectroscopy and the spectrum ultraviolet UV to investigate about the compound and functional groups in this extract. Use of this extract represents a new method for chromosome banding and a viable alternative to existing chromosome staining methods.
Highlights
A wide variety of stains are used to visualise chromosomes under the microscope
The aceto-orcein, acetocarmine, gentian violet, and haematoxylin readily stain chromatin are classical cytological stains allowing for easy visualisation of chromosomes under a standard light microscope [1]
Each chromosome arm is divided into regions, or cytogenetic bands, that can be seen using a microscope and special stains
Summary
A wide variety of stains are used to visualise chromosomes under the microscope. Numerous methods are available for identifying chromosomes and preparing karyotypes for clinical and research purposes. The most common methods of dye-based chromosome banding are Giemsa- (G), reverse- (R), centromere- (C), and quinacrine- (Q) banding.
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