Abstract
AbstractA detailed method is described for encapsulating large segments of fixed primate brain tissue in formalin-hardened egg yolk. The procedure imparts advantages of en bloc embedding to the frozen section technic, without shrinkage, infiltration by foreign substances or chemical alteration of the tissue. Proper anatomical relationships of noncontiguous portions of cerebrum, cerebellum, brainstem and cranial nerves are accurately preserved in free floating sections; and exact positioning on microslides is easily accomplished before or after staining. The method is compatible with staining, histochemical and autoradiographic technics normally performed on frozen sections of fixed brain tissue.
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