Abstract
Ovotransferrin, a member of the transferrin family, is the second main protein found in egg white. Ovotransferrin was reported to have antimicrobial, antioxidant, and immunomodulating activities. The aim of this work was to characterize the cellular and molecular functions of egg white ovotransferrin on osteoclasts differentiation and function. Osteoclasts were prepared from mouse macrophage RAW 264.7 cells stimulated with receptor activator of nuclear factor κB ligand (RANKL). Ovotransferrin inhibited osteoclasts differentiation and the calcium–phosphate resorptive ability via the suppression of RANKL-induced nuclear factor κ-light chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways. Ovotransferrin induced apoptosis of matured osteoclasts, accompanied by increased expression of Bcl-2-like protein 11 (Bim) and Bcl-2-assoicated death promoter (Bad), but decreased expression of B-cell lymphoma 2 (Bcl-2) and B-cell lymphoma-extra-large (Bcl-xl). We established a novel role of egg white ovotransferrin as an inhibitor of osteoclastogenesis, which may be used for the prevention of osteoporosis.
Highlights
Bone is a dynamic organ that undergoes continuous remodeling to change its mass and form [1].When healthy, bone remodeling maintains a delicate balance, in which the amounts of total bone resorption regulated by osteoclasts and total bone formation regulated by osteoblasts remain the same [2]
We established a novel role of egg white ovotransferrin as an inhibitor of osteoclastogenesis, which may be used for the prevention of osteoporosis
We demonstrated that ovotransferrin exerts an anti-osteoclastogenesis effect via suppressing RANKL activation of NF-κB and mitogen-activated protein kinase (MAPK) signaling pathways
Summary
Bone is a dynamic organ that undergoes continuous remodeling to change its mass and form [1].When healthy, bone remodeling maintains a delicate balance, in which the amounts of total bone resorption regulated by osteoclasts and total bone formation regulated by osteoblasts remain the same [2]. Bone is a dynamic organ that undergoes continuous remodeling to change its mass and form [1]. Over-activated osteoclasts activity can lead to osteolytic bone conditions, resulting in several bone diseases such as osteoporosis [2]. The inhibition of osteoclastic activity and the resultant bone resorption have a profound effect on regulating abnormal remodeling processes and is therapeutically important for osteoporosis prevention. Osteoclasts, which function to resorb bone, are giant and multinucleated cells derived from monocytic and macrophage lineage [4]. The generation and differentiation of osteoclasts from their precursors are crucial. The essential step in osteoclasts generation is the binding of the receptor-activator of nuclear factor κ-light chain-enhancer of activated B cells (NF-κB) ligand (RANKL)
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