Egg white hydrolysate improves fatigue due to short-term swimming load test in mice.

Ryosuke Matsuoka,Shinya Uno,Hiroyuki Shidara,Masaaki Kunou,Mamoru Kimura

doi:10.1002/fsn3.810

Abstract

We studied the effect of egg white hydrolysate (EWH) on swimming endurance in mice. 7‐week‐old male ddY mice (28–30 g) were divided into three groups and fed an AIN‐93G diet supplemented with casein (n = 8), EWH (n = 7), or egg white protein (EWP, n = 8) for 14 days. From day 11, the mice underwent a swimming test daily with a weight load equivalent to 10% of their body weight, and the lengths of time they swam were recorded. Blood was sampled for testing on the last day of the study. We observed that increases in the swimming duration through day 14 were significantly greater in the EWH group than in the casein group (p = 0.049). As a factor underlying this, the hexanoyl‐lysine level in blood was confirmed to be decreased in the former group (p = 0.013). These findings indicate that consumption of EWH extended the swimming duration and suggest the mechanistic involvement of an antifatigue effect mediated by its antioxidant activity.

Highlights

A significant positive correlation between protein intake and muscle mass has been reported (Houston et al, 2008)
At the commencement of the study, the mean swimming time was 132 ± 16 s in the casein group, 151 ± 21 s in the egg white hydrolysate (EWH) group, and 151 ± 21 s in the Egg white proteins (EWP) group, showing no significant differences among the three groups
The results of this study confirmed that EWH exhibited an antifatigue effect via its antioxidant activity

Summary

| INTRODUCTION

A significant positive correlation between protein intake and muscle mass has been reported (Houston et al, 2008). Strenuous exercise produces excessive free radical in muscle tissues (Murakic-Sposta et al, 2015), while causing muscles to be damaged or fatigued (Nosaka & Newton, 2002; Nosaka, Newton, & Sacco, 2002). This can lead to reduced physical activities, which may turn into a vicious cycle of muscle mass decline. BCAAs have been reported to ameliorate muscle fatigue (Matsumoto et al, 2009) Their concentration in the blood increases after ingesting an EWP preparation and decreases after exercise (Kato, Numao, Miyauchi, & Suzuki, 2010). There are no reports on the antifatigue effects of proteins, such reports have been published for egg white-derived peptides. We assessed the influence of the EWH preparation on swimming endurance in mice

| MATERIALS AND METHODS

| DISCUSSION

Findings

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