Egg Storage and the Embryo

Abstract

In domestic avian species, eggs are stored at cool temperatures until they can be placed into an incubator. The low temperature-induced diapause enables the embryo to survive until optimal temperature and humidity incubation conditions can be provided to support embryonic growth. Egg storage is a logistical necessity for the hatching egg industry both at the breeder farm and at the hatchery. However, it is well known that egg storage longer than 7 d negatively influences hatchability. At the cellular level, long-term egg storage induces cell death. This appears to be occurring both via necrosis and apoptosis. The result is higher embryonic mortality and, consequently, lower hatchability. In addition, long-term egg storage influences embryonic development and metabolism. Embryos of eggs stored long-term can be affected such that they do not initiate growth after proper incubation temperatures are provided; they initiate growth, but grow at a slower rate than eggs stored short term; and they are affected in both of the previously mentioned ways. Development of equipment to measure the embryonic metabolism of individually incubating eggs over the entire 21 d of incubation has provided further evidence that embryo metabolism has changed due to storage. One of the methods to reduce the negative effects of long-term storage has been to incubate eggs for short periods before storage. In both turkey and chicken eggs, this technique has been successful in improving the hatchability of long-term stored eggs. It is hypothesized that particular embryonic developmental stages are better able to survive long-term storage. Future research should focus on the mechanisms behind this improved ability to survive storage.