Abstract
BackgroundThe purpose of the present study is to investigate the direct biological effects of the epidermal growth factor receptor (EGFR) inhibitor C225 on the radiosensitivity of human lung squamous cancer cell-H520. H520 cells were treated with different dosage of 60Co γ ray irradiation (1.953 Gy/min) in the presence or absence of C225. The cellular proliferation, colony forming capacity, apoptosis, the cell cycle distribution as well as caspase-3 were analyzed in vitro.ResultsWe found that C225 treatment significantly increased radiosensitivity of H-520 cells to irradiation, and led to cell cycle arrest in G1 phase, whereas 60Co γ ray irradiation mainly caused G2 phase arrest. H-520 cells thus displayed both the G1 and G2 phase arrest upon treatment with C225 in combination with 60Co γ ray irradiation. Moreover, C225 treatment significantly increased the apoptosis percentage of H-520 cells (13.91% ± 1.88%) compared with the control group (5.75% ± 0.64%, P < 0.05).ConclusionIn this regard, C225 treatment may make H-520 cells more sensitive to irradiation through the enhancement of caspase-3 mediated tumor cell apoptosis and cell cycle arrest.
Highlights
It is well known that many non-small cell lung cancer (NSCLC) cells over-express membrane surface epidermal growth factor receptor (EGFR) [1,2,3,4,5,6,7]
High expression of EGFR in H-520 cells In order to determine the expression of EGFR in our cell line H520, we firstly performed indirect staining to detect EGFR expression with fluorescence-activated cell sorting (FACS)
The percentage of EGFR expression in normal lung tissue cells is below 10%
Summary
It is well known that many non-small cell lung cancer (NSCLC) cells over-express membrane surface epidermal growth factor receptor (EGFR) [1,2,3,4,5,6,7]. In the in vitro studies, C225 combined with radiotherapy or chemotherapy inhibits the growth of head and neck squamous carcinoma cells dramatically. In the clinical treatment of head and neck squamous cell carcinoma [17,18], C225 combined with radiotherapy has made a fantastic therapeutic effect [14,15]. The purpose of the present study is to investigate the direct biological effects of the epidermal growth factor receptor (EGFR) inhibitor C225 on the radiosensitivity of human lung squamous cancer cell-H520. The cellular proliferation, colony forming capacity, apoptosis, the cell cycle distribution as well as caspase-3 were analyzed in vitro
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