EGFL7-blocking antibody inhibit pituitary adenoma proliferation and invasion

Abstract

Objective: To study the effect of EGFL7-blocking monoclonal antibody inhibiting the proliferation and invasion of mouse adrenocorticotropic hormone (ACTH)-secreting pituitary adenomas (ACTHomas) AtT-20 cells. Methods: After treated with different concentration of EGFL7-blocking monoclonal antibody for 24, 48, 72 h, the proliferation of AtT-20 cells was determined by MTS. Simultaneously, ACTH secretion of AtT-20 cells by ELISA and the mRNA expression of invasion related genes such as E-cadherin, snail, MMP-2 and MMP-9 by Real-time PCR were detected. Results: EGFL7-blocking monoclonal antibody could effectively inhibit the proliferation and ACTH secretion of AtT-20 cells in a dose-dependent and time-dependent manner. At the same time, EGFL7-blocking monoclonal antibody significant down-regulated the mRNA level of snail, MMP-2 and MMP-9 in AtT-20 cells. Conclusion: EGFL7-blocking monoclonal antibody could inhibit mouse pituitary adenoma AtT-20 cells proliferation and invasion.