Abstract
Objective To investigate the effects of sevoflurane and scopolamine on muscarinic acetylcholine receptors in hippocampus in gerontal rata.Methods Forty-eight male SD rats aged 18 months weighing 560-600 g were randomly divided into 3 groups(n=16 each):group Ⅰcontrol(C);group Ⅱ inhaled 3%sevoflurane for 2 h(Sevo);group Ⅲ received intraperitoneal scopolamine 0.8 mg/kg.Y-maze was used to test the abihty of learning and memow at 1 and 3 d after drug administration in 8 animals from each group.The animals were decapitated immediately after Y-maze test at 1 and 3 day after drug administration.The hippocampus was removed for determination of the relative levels of expression of M1 and M2 acetylcholine receptor(mAChR M1,M2)mRNA(by RT-PCR)and the expression of mAChR MI and M2(by immuno-histochemistry).Results The ability to learn and remenlber and the expression of M1 and M2 AChR and AChR mRNA were significantly decreased at 1 d after sevoflurane inhalation(in group Ⅱ)and scopolamine IP administration(in group m)aS compared with control group and returned to control level at 3 d.Conclusion The ability to learn and remember cfln be reduced for a short period of time after sevoflurane inhalation and IP scopolamine throgh down-regulation of Ml and M2 mAChR in the brain. Key words: Anesthetics,inhalation; Scopolamine; Cognition disorders; Hippocampus; Aged; Receptor,muacarinic M1; Receptor,muscarinic M2
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