Effluent solids recirculation to municipal sludge digesters enhances long-chain fatty acids degradation capacity

Sepehr Shakeri Yekta,Thuane Mendes Anacleto,Luka Šafarič,Mette Axelsson Bjerg,Anna Schnürer,Anna Karlsson,Annika Björn,Xavier Goux,Tong Liu

doi:10.1186/s13068-021-01913-1

Abstract

BackgroundSlow degradation kinetics of long-chain fatty acids (LCFA) and their accumulation in anaerobic digesters disrupt methanogenic activity and biogas production at high loads of waste lipids. In this study, we evaluated the effect of effluent solids recirculation on microbial LCFA (oleate) degradation capacity in continuous stirred-tank sludge digesters, with the overall aim of providing operating conditions for efficient co-digestion of waste lipids. Furthermore, the impacts of LCFA feeding frequency and sulfide on process performance and microbial community dynamics were investigated, as parameters that were previously shown to be influential on LCFA conversion to biogas.ResultsEffluent solids recirculation to municipal sludge digesters enabled biogas production of up to 78% of the theoretical potential from 1.0 g oleate l−1 day−1. In digesters without effluent recirculation, comparable conversion efficiency could only be reached at oleate loading rates up to 0.5 g l−1 day−1. Pulse feeding of oleate (supplementation of 2.0 g oleate l−1 every second day instead of 1.0 g oleate l−1 every day) did not have a substantial impact on the degree of oleate conversion to biogas in the digesters that operated with effluent recirculation, while it marginally enhanced oleate conversion to biogas in the digesters without effluent recirculation. Next-generation sequencing of 16S rRNA gene amplicons of bacteria and archaea revealed that pulse feeding resulted in prevalence of fatty acid-degrading Smithella when effluent recirculation was applied, whereas Candidatus Cloacimonas prevailed after pulse feeding of oleate in the digesters without effluent recirculation. Combined oleate pulse feeding and elevated sulfide level contributed to increased relative abundance of LCFA-degrading Syntrophomonas and enhanced conversion efficiency of oleate, but only in the digesters without effluent recirculation.ConclusionsEffluent solids recirculation improves microbial LCFA degradation capacity, providing possibilities for co-digestion of larger amounts of waste lipids with municipal sludge.

Highlights

Slow degradation kinetics of long-chain fatty acids (LCFA) and their accumulation in anaerobic digesters disrupt methanogenic activity and biogas production at high loads of waste lipids
As a result of effluent recirculation, total solids (TS) content in R4 increased from 2.6 ± 0.2% of total weight during the start-up phase to 4.6 ± 0.2% towards the end of the experiment, whereas the relative volatile solids (VS) content declined from 56 ± 2 to 43 ± 1% of TS (Table 2)
Effluent recirculation led to higher average daily biogas production by ~ 10%, a higher TS content with a larger proportion of inorganic fractions, and higher pH by ~ 0.2 units during the Primary and activated sewage sludge (PASS) digestion

Summary

Introduction

Slow degradation kinetics of long-chain fatty acids (LCFA) and their accumulation in anaerobic digesters disrupt methanogenic activity and biogas production at high loads of waste lipids. Anaerobic digester units at wastewater treatment plants (WWTP) account for Shakeri Yekta et al Biotechnol Biofuels (2021) 14:56 approximately half of the existing biogas production facilities in Sweden and their existing capacity can be used for more biogas production through co-digestion of energy-rich organic wastes [3, 4]. In this context, waste lipids are regarded as attractive co-substrates because of their high methane potential and energy density [5]. The low hydrogen partial pressure is established by syntrophic association of LCFA-degrading bacteria with hydrogen- and/or formate-utilizing microorganisms in anaerobic digesters, where co-occurrences and activity of β-oxidizers (e.g., families Syntrophomonadaceae and Syntrophaceae) together with hydrogenotrophic methanogens (e.g., Methanoculleus, Methanobacterium, and Methanothermobacter) have been identified as the main contributors to LCFA degradation [9]

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Results

Discussion

Conclusion