Abstract
The atmospheric and room temperature plasma (ARTP) mutagenesis technique was first used in the present study to produce whole-genomic mutants in blunt snout bream (Megalobrama amblycephala). The optimal ARTP mutation parameters for semen were established according to sperm motility and abnormal embryonic rate. A total of 2026 mutated offsprings were produced with normal and abnormal individuals with a ratio of approximately 1:1. Among them, 384 normal mutant fish (~20%), after three months of cultivation, demonstrated improved performance with either fast growth or hypoxia tolerance, and 89 mutant individuals with both hypoxia tolerance and fast growth (H&F). The average bodyweight of the H&F fish increased by 26.48% compared with the control group. The average oxygen tension threshold for loss of equilibrium (LOEcrit) of the H&F fish was 0.45 mg/L, significantly (p < 0.05) lower than 0.86 mg/L in the control group. Meanwhile, the red blood cell count, hemoglobin concentration, and Na+/K+-ATPase enzyme activity of the H&F group increased significantly (p < 0.05), whereas, the gill tissue cell apoptosis rate decreased considerably (p < 0.05), suggesting that they are hypoxia tolerant. According to the genome resequencing analysis, 3,195,434 SNPs and 927,930 InDels were found in the H&F group, which were significantly higher than the 9127 SNPs and 2908 InDels in the control group. The average mutation rate in the H&F fishes reached 2.98 × 10−3 at the genome level, approximately 350 times that of the control group. Importantly, 3651 special non-synonymous SNP mutations were found in the H&F fish, which are located in 1223 genes. Among them, eight hypoxia- and growth-related genes with non-synonymous mutations at one or more sites were differentially expressed (DE) in H&F fish. Taken together, our results establish a novel whole-genomic mutagenesis approach using ARTP in blunt snout bream, which should be useful for future genetic breeding.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
