Efficient synthesis of γ-methyl-capped guanosine 5′-triphosphate as a 5′-terminal unique structure of U6 RNA via a new triphosphate bond formation involving activation of methyl phosphorimidazolidate using ZnCl 2 as a catalyst in DMF under anhydrous conditions

Abstract

P 1-Methyl P 3-(5′-guanosyl) triphosphate (CH 3pppG), which exists as a unique structure at the 5′-terminal region of U6 RNA, was synthesized in an excellent yield by methyl phosphorimidazolidate ( 2c) with the bis(tetrabutylammonium) salt of GDP in the presence of ZnCl 2 as an effective catalyst in dry DMF.