Abstract
The amyloidogenic Aβ42 peptide was efficiently prepared using a double linker system, markedly improving solubility and chromatographic peak resolution, thus enabling full characterisation using standard techniques. The tag was readily cleaved with sodium hydroxide and removed by aqueous extraction, affording Aβ42 in high purity and yield for biophysical characterisation studies.
Highlights
The amyloidogenic Aβ42 peptide was efficiently prepared using a double linker system, markedly improving solubility and chromatographic peak resolution, enabling full characterisation using standard techniques
Endogenous Aβ peptide is derived from enzymatic processing of the transmembrane Amyloid Precursor Protein (APP) by the secretase family of enzymes
It is understood that in the disease state, there is a preferential shift towards the latter process, which results in the excessive production of Aβ peptide, leading to its gradual aggregation into plaques
Summary
Efficient synthesis and characterisation of the amyloid beta peptide, Aβ1–42, using a double linker system† The amyloidogenic Aβ42 peptide was efficiently prepared using a double linker system, markedly improving solubility and chromatographic peak resolution, enabling full characterisation using standard techniques.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have