Abstract
A two-step white rot–brown rot fungal pretreatment of corn cobs was proposed to enhance enzymatic hydrolysis efficiency while reducing the lengthy time requirement for the conventional fungal treatment process. Corn cobs were first pretreated by white rot fungi for 25 days, followed by short time brown rot fungi treated for 7 days. The results indicated that the enzymatic hydrolysis efficiency was dramatically improved as a result of the synergistic effects of combined pretreatments. The highest glucose yield of 83.0% was obtained by Trametes orientalis (Cui6300)–Fomitopsis pinicola (Cui12330) pretreatment. Chemical composition and FT-IR observations demonstrated that hemicelluloses were selectively degraded. Additionally, SEM analysis illustrated that the structural disruption of pretreated corn cobs occurred and might account for the enhancement of the hydrolysis efficiency. In this processing, 7 days of brown rot fungal treatment played a significant role in enzymatic hydrolysis enhancement. Cellulose ...
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