Abstract
Passion fruit (Passiflora edulis(Sims)) is currently ranked third among fruit exports from Kenya and has great potential since the demand for both fresh fruit and processed juice is on a continuous increase. Passion fruit production in Kenya is constrained by a lack of healthy, clean planting material, poor seed viability, and low germination rates. To address this, the present study reports anin vitroplant regeneration protocol for passion fruit using leaf disc and nodal explants and genetic fidelity analysis of the regenerated plants. The highest number of shoot regeneration was obtained on Murashige and Skoog (MS) medium supplemented with 2 mg·L−16-Benzyl amino purine (BAP) (shoot induction medium). The multiplication of shoots was optimum in MS medium supplemented with 3 mg·L−1BAP. To eliminate the requirement of an additional step ofin vitrorooting, exogenous application of putrescine induced the formation and development of roots on nodal explants. Genetic fidelity analysis of thein vitroregenerated and macropropagated plants with that of the mother plant was carried out by sequence-related amplified polymorphism (SRAP) markers, and monomorphic banding profile for 80% of the regenerants confirmed the genetic uniformity of thein vitroregenerated and macropropagated plants. Thein vitroregeneration system developed can be utilized for mass clonal propagation for the economic commercial exploitation of this important tropical fruit.
Highlights
Passion fruit (Passiflora edulis Sims) is an economically important perennial fruit crop in many tropical and subtropical countries, mainly grown for its edible fruit and ornamental and medicinal use [1,2,3]
Among the diseases limiting passion fruit production is woodiness disease (PWD)-complex which is mainly transmitted by aphids. is is a serious disease in all passion fruit production areas in Kenya, affecting both the purple and the yellow forms [8, 9]. e disease has been reported to cause up to 100% loss in fruit yields in Kenya [10]. e mode of propagation entails the use of seed and grafting which has led to the build-up of diseases especially of the woodiness virus complex resulting in total yield losses and lack of clean planting materials
Morphogenetic responses of leaf disc explants were observed on Murashige and Skoog (MS) medium supplemented with Benzyl amino purine (BAP) irrespective of the concentration of BAP tested. e first morphogenetic responses were visible after 21 days of culture when shoot buds were observed from the leaf explant (Figure 1)
Summary
Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulis Sims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers. The present study reports an in vitro plant regeneration protocol for passion fruit using leaf disc and nodal explants and genetic fidelity analysis of the regenerated plants. E highest number of shoot regeneration was obtained on Murashige and Skoog (MS) medium supplemented with 2 mg·L−1 6-Benzyl amino purine (BAP) (shoot induction medium). Genetic fidelity analysis of the in vitro regenerated and macropropagated plants with that of the mother plant was carried out by sequence-related amplified polymorphism (SRAP) markers, and monomorphic banding profile for 80% of the regenerants confirmed the genetic uniformity of the in vitro regenerated and macropropagated plants. Genetic fidelity analysis of the in vitro regenerated and macropropagated plants with that of the mother plant was carried out by sequence-related amplified polymorphism (SRAP) markers, and monomorphic banding profile for 80% of the regenerants confirmed the genetic uniformity of the in vitro regenerated and macropropagated plants. e in vitro regeneration system developed can be utilized for mass clonal propagation for the economic commercial exploitation of this important tropical fruit
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