Abstract
We made the protoplast cell of red cabbage. And we irradiated to the protoplast cell with continues-wave laser. It is impossible to break down the protoplast cell. On the other hand we use the slide evaporated gold thin film, it is possible to break down a few protoplast cell. We use the femtosecond laser, it is possible to break down almost protoplast cell. The femtosecond laser used in experiment is mode-locked fiber ring laser. It also has 300fs pulse width, 52kW peak power and 4.773MHz pulse repetition frequency. Experiments show it is possible to break down the protoplast cell on an electrode.
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